htpX [2017-8-11 16:0:3]

stress-responsive membrane protease

locus: BSU13490

pI: 10.16

mw: 32.71 kDa

protein length: 298 aa

gene length: 894 bp

function: quality control of membrane proteins

product: membrane protease

essential: no

synonyms: ykrL

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,414,997 → 1,415,893

Phenotypes of a mutant

• increased sensitivity to membrane protein overproduction [Pubmed|22447908]
• increased sensitivity to dissipation of the membrane potential by the addition of valinomycin [Pubmed|22447908]

The protein

Protein family

• peptidase M48B family (according to Swiss-Prot)

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|62B6875301B458F04E3F65717AD3A5925A9C4973|htpX]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|7283CDE72293A68D0B35E0C028D22FABE7FB43C7|YkrK]: repression, [Pubmed|22447908], in [regulon|7283CDE72293A68D0B35E0C028D22FABE7FB43C7|YkrK regulon]
  • [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]: repression, [Pubmed|22447908], in [regulon|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok regulon]

  regulation

  • expression is increased due to mutations in ''[protein|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|ResE]'', ''[protein|495721E4B8BF6FEC01E62E86339560F90776EED1|ResB]'', ''[protein|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|SdhC]'', and'' [protein|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|SdhA]'' [Pubmed|22447908]

Biological materials

Mutant

• MGNA-B316 (ykrL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1315 NBRP B. subtilis, Japan]

References

• 22447908,22624725,22383849,23042994,26883633